Using genome walking PCR, left flanking sequence of the T-DNA integration site on rice genome was am-plified. T-DNA integration site on rice genome was determined by nucleotide blast using left flanking sequence asquery. The results showed that T-DNA was inserted into the position 41 607 441 bp of chromosome 1（Gen Bank se-quence ID: NC₀08394.4）. Right flanking sequence of the T-DNA integration site was also amplified by using T-DNA right border specific primers and right primer of insertion site. Based on the left flanking sequence and inser-tion site, the T-DNA left border specific primer and insertion site specific primers on‘Jishengjing 2’genome weredesigned. Using the primers, the event-specific detection PCR for‘Jishengjing 2’was established. It will providethe effective method for identification of‘Jishengjing 2’.