In the culture experiment, tree leaves, rice chaff, crop straw were mixed the fertilizer and applied intosoil, their effect on content and form of Pb was studied. The results showed that application of natural organic materi-als made the total content of Pb and the content of all forms of Pb in soil increased. The increase in rice chaff treat-ment was the largest. The content of acid extractable of Pb and reducible Pb increased, but oxidation states of Pband the residual state of Pb reduced. So Pb in soil changed from low bioavailability fraction to high bioavailabilityfraction. The changes in rice chaff treatment was the largest. The content of organic matter and p H decreased gradu-ally. The relationship between the content of various forms of Pb and organic matter in all treatments was significant-ly more than the relationship with pH.