In order to investigate the molecular mechanisms underlying porcine intramuscular fat deposition, thetranscriptional profile of longissimus dorsi muscle between lower intramuscular fat content individuals and higher in-dividuals were compared by microarrays in Songliao Black Swine, and the differentially expressed genes were veri-fied by real- time PCR. The results showed that 153 differentially expressed genes were found between the twogroups. Differentially expressed genes classified by GO（Gene Ontology） analysis mainly involved in cell growth anddeath, signaling molecules and interaction, signal transduction, metabolism of amino acids, lipid metabolism etc. Inaddition, the Real-time-PCR results of IGF2、PRKAG3、UCP3 were consistent with that of gene chip. These resultswould offer the basic data for the further study on the molecule genetic mechanism of intramuscular fat deposition inSongliao Black Swine.