The large-scale screening and evaluation technology of maize DH lines was preliminary assembled andintegrated according to many years’ corn breeding experience and evaluation researches of maize DH lines in do-mestic and abroad. Special steps, such as propagation of DH lines, screening and test-crossing were explained inthe paper. After many years of practice and verification, it was proved that maize DH lines can be selected effective-ly by large scale application of this process.