To investigate the anti-fatigue effect of Lactobacillus plantarum C88 combined with Acanthopanax sessiliflorus polysaccharide（ASP）, 88 Kunming mice were divided into 4 groups, and received, ASP, L. plantarum C88 and L. plantarum C88+ASP respectively by gavage. The time of forced swimming fixed, swimming exhausted timeand biochemical indicators of liver and serum were determined by kits. The results indicated that ASP and L. plantarum C88 could shorten forced swimming fixed time and extend the exhaustive swimming time of mice. The reservesof liver glycogen and the levels of superoxide dismutase（SOD） and catalase（CAT） increased, while lactic acid（LD）,blood urea nitrogen（BUN） and malondialdehyde（MDA） levels in serum reduced significantly. The effects of ASPcombined with L. plantarum C88 was significantly stronger than that treated by ASP or L. plantarum C88 alone. Inconclusion, ASP combined with L. plantarum C88 presented certain anti-fatigue effects.