PCR was used to investigate the distribution of 5 ARGs, including sul1, sul2, tetA, tetB and tetC in ground-water and farmland soil of Jiaxing City. The results showed that all these five genes were all detected in both waterand soil samples. The detection rate of sul1, sul2, tetA, tetB, tetC was 65.0%, 95.0%, 80.0%, 40.0% and 90.0%,based on 20 groundwater samples and 55.6%, 100.0%, 55.6%, 44.4% and 88.9% based on 9 farmland soil samples,respectively. The results revealed that Jiaxing City was widely contaminated with ARGs, drawing higher demand inwater pollution control of Jiaxing City.