579 bacteria strains were isolated from rice rhizosphere soil in 16 counties of Heilongjiang Province byway of streak plate method. A strain of Sc2 was finally selected to have strong inhibitory activity against Magnaporthe oryzae with the inhibition rate of 73.44% by plate confrontation method screening. Combined with its colonymorphology, biochemical characteristics and 16 s rDNA sequence analysis, the strain Sc2 was confirmed to be Bacillus subtilis. The Sc2 had strong inhibitory activity against the hypha growth and spore germination of M. oryzea. Withthe increase of dilution ratio, inhibitory activity was gradually decline. The inhibition rate of spore germination after24 h and hypha growth on 7 d was more than 60% with 40 times diluent of 1.8×108cfu/m L fermentation liquid. Re-sults of indoor pot experiment showed that the Sc2 prevention was mainly in the prevention of rice blast. Preventiveefficacy was 90.33% when Sc2 was mixed with tricyclazole by 1:2.