Objective of the study was establishing the HPLC fingerprint of two kinds of edible rose, Dianhong rose and crimson glory rose, and to provide reliable methods for scientific evaluation. Ten batches of each edible rose were determined by HPLC, and analyzed by"Evaluation on Similarity of Traditional Chinese Medicine Chromatographic Fingerprint"2004 A system. The standard fingerprints of two kinds of edible roses were established and they were accurate for cultivar identification.