For screening of soybean varieties tolerant to iron deficiency in calcareous soil, the tolerance of 126 soy-bean varieties to iron deficiency were identified by cluster analysis according to chlorophyll content and biomass perplant at V3 and R2 stages in calcareous soil area of Taonan, Jilin Province. The results showed that the differencesof both chlorophyll content and biomass per plant at different stages between soybean varieties were significant. Onehundred twenty six soybean varieties were divided into 5 levels, eighteen varieties in Level 1, accounting for 14.3%of testing varieties, their tolerance to iron deficiency was the strongest for their higher chlorophyll content and bio-mass per plant during whole growth period; thirty three varieties were in Level 2, accounting for 26.2% of testing va-rieties, stronger tolerance, lower biomass per plant than that of Level 1, thirty varieties in Level 3, twenty three pointeight percent of testing varieties, moderate tolerance, leaf chlorosis at the early stage, though recovered later, but drymatter accumulation at later stage was affected; twenty seven varieties in Level 4, twenty one point four percent oftesting varieties, less sensitive to iron deficiency, leaf chlorosis during whole growth period, dry matter accumulationwas significantly affected, eighteen varieties in Level 5, fourteen point three percent of testing varieties, sensitive toiron deficiency, leaf chlorosis was severe at the early stage, and died later.