A large amount of the chemical agent for seedling strengthening applied for a long time had caused a seri-ous environmental pollution in arid rice nursery fields. In this paper, using the method of field experiment, severalfunctional microbial bacteria were mixed with mineral nutrition to make a compound microorganism bacteriumagent, and it was applied to the rice seedlings. The activity of superoxide dismutase（SOD）, peroxidase（POD）, phe-nylalnine ammonialyase（PAL）, the content variation of proline（Pro） and malondialdehyde（MDA） during the riceseedling stage were determined. The results showed that the activity of SOD, POD, PAL and the content of prolineincreased significantly, and MDA content decreased obviously. The enhance of seedling stress resistant enzyme isone important reason of the compound microbial fertilizer to raise stress resistance of rice seedlings.