The aerial conidias, in vitro blastspores and submerged conidias of Beauveria bassiana D1-5 strain wereobserved by electron microscope, respectively, their specific expression protein were analyzed and screened via two-dimensional electrophoresis. The results indicated that aerial conidias was spherical, packaged by rod like structure,gave an obviously hydrophobic property. In vitro blastspore showed cylindrical, unequal in size, and smooth surface.Submerged conidia was transparent sphere, equal in size, and a rough surface, the wall of three kinds of sporesshowed a similar thickness. PDquest software was used to analyze data, after isolation of specific expression proteinof three kinds of spores by two-dimensional electrophoresis. Comparison of in vitro blastspores and submerged coni-dias indicated that there were 8 up-regulated and 12 down-regulated proteins among 20 points gave more than twotimes change. Comparison of aerial conidias and in vitro blastspores indicated that there were 43 up-regulated and 14 down-regulated proteins among 57 points gave more than two times change. Comparison of aerial conidias andsubmerged conidias indicated that there were 8 up-regulated and 58 down-regulated proteins among 66 points gavemore than two times change.