Effect of different low temperature on physiological and biochemical indexes of corn had been studied fortimely sowing in seed germination and seedling growth. Six average temperatures were designed as 4℃, 6℃, 8℃,10℃, 12℃ and 15℃,and two varieties of corn‘Xianyu 335’and‘Zhengdan 958’were used. The result showedthat corn seed germination and seedling growth were damaged under 8℃. Soluble protein content had no obviouschange in corn seed, MDA content and POD activity increased, but SOD activity and CAT activity dropped rapidlyunder 8℃. Corn seed could not grow and develop normally above 10℃. Soluble protein content was reduced in cornseed with the increase of temperature. Seed germinating rate at 15℃ was better than at 12℃.