In this paper, a gfp gene were introduced into the rice sheath blight fungus by agrobacterium mediated transformation and its effects on the stability and pathogenicity of the fungus investigated. The results showed that30μg/m L of selection agent hygromycin B could significantly inhibit the growth of Rhizoctonia solani on PDA medium. The optimized parameters for A. tumefaciens mediated transformation of Rhizoctonia solani were as follows: 250μmol / m L of acetosyringone（AS） during the co-culture, pre-inducement time for 9 h, culture temperature at 26 ℃,p H 5.8~6.0 during co-culture and the total culture time for 22 h. About 5 randomly selected transformants were analyzed by fluorescence microscope, sequencing and PCR. The results indicated that the gfp gene was transformed into Rhizoctonia solani. The stability and pathogenicity analysis of the transformed fungus showed that 5 transformants after continuous culture for five generations still showed resistance to the selection agent hygromycin B, confirming the stable inheritance of the gfp gene in the transformed rice sheath blight fungus. The transformed fungus exhibited similar disease severity as that of the wide type strain after inoculating rice, indicating that the pathogenicity of the Rhizoctonia solani was not significantly changed by introduction of the exogenous gfp gene. Our results would lay the foundation for further investigation on the interaction mechanism between Rhizoctonia solani and rice varieties.