To clarify the resistance of Botrytis cinerea of strawberry in Xuzhou to procymidone, the mycelium growth rate method was used to test the resistance to procymidone at distinctive concentrations in 93 isolates of B. cinerea from 8 counties of Xuzhou between 2014 and 2015. The results showed that the resistance frequency of B. cinerea in Xuzhou to procymidone was 54.83%, and middle-level resistance frequency to procymidone was 34.40%. The resistance frequency of isolates from Tongshan District, Jiawang District and Pei County all exceeded 70%. The middle-level resistance to procymidone of B. cinerea from strawberry in Xuzhou was common. The procymidone should be replaced or rotated in strawberry production in Xuzhou.