The hot pepper male sterile lines O-6 and O-9 was used as experimental materials and they were sowed at interval. In full flowering stage, changes of the content of soluble sugar, soluble protein and proline were deter-mined at 5 development stage, i.e., the stage of sporogenous cells, the stage of microspore mother cells, the stage of tetrads, the stage of monoclear microspores and the stage of pollen grains.The results showed that when sowing date was postponed, the content of soluble sugar, soluble protein and proline in anthers of the same growing period decreased. The highest content was at the stage of sporogenous cells. But the content of soluble sugar in flowers at thesame sowing date increased firstly and decreased at last and the highest content was at the stage of tetrads, O-6 was 2.17 mg/g·FW, O-9 was 1.23 mg/g·FW.