To clarify main influencing factors under long-term continuous maize cropping obstacle, research has been carried out according simulation for field continuous cropping. The result showed that root exudates, decomposed substance, air dry matter, root extract and benzoic acid of low concentration had no more distinct effect on physiological and growth traits of maize seedling. But, these substances of high concentration had strong development inhibition to physiological and growth traits in maize seedling. In a word, maize seedling growing was markedly inhibited with the number of continuous maize cropping years increased.