Based on the field experiment of winter and spring pepper as well as autumn and winter tomato planted in greenhouse, this experiment was to study effect of combinatory application of biogas manure and chemical fertilizer on the growth and development of vegetables. The yield, soluble solids, Vc and total acids of the pepper and tomato were measured, and the physiological index of the pepper such as main root length, lateral root length, root volume,plant height, stem diameter, and plant expansion determined. The results demonstrated that combinatory application of biogas manure and chemical fertilizer effectively promoted the root development and plant growth of vegetable crops, raised the yield of pepper and tomato, and improved the quality of vegetable products.