Using pre-column derivatization and high performance liquid chromatography（HPLC） method to determine the content of gamma-aminobutyric acid（GABA） in different parts of Cerasus humilis. The chromatographic conditions adopt the C18 chromatographic column（4.6 mm × 250 mm, 5 microns） with acetonitrile -0.1% acetic acid solution as mobile phase and the detection wave length of 254 nm. GABA showed a good linear relationship in the linear range inside 5-100μg·mL^-1. Results showed that the content of GABA in root, stem, leaf, bud, flower, flower buds, fruits were really different, and it is the highest in the stem and the lowest in the root, which is 912.9μg·mg^-1and 263.3μg·mg^-1, respectively. This method is easy to operate, meanwhile it has stable results and is really suitable for determining the content of GABA in different parts of the C. humilis, which provides the basis in the comprehensive development of the C. humilis.