In this paper, main factors affecting the survival rate of plant lets were analyzed, and the transplanting techniques to improve the survival rate of somatic cell regenerated and tissue cultured seedlings were summarized.Main factors affecting the survival rate of tissue cultured seedling were the development condition of the tissue culture seedlings, the acclimation of the plantlets, the selection of the transplanting substrate, the control of the transplanting conditions, and the grafting technology. During the acclimation period, we should gradually change sealing parafilms and reduce the moisture in culture bottles to promote lignification of seedlings. During grafting of plantlets, a cotyledon should be retained on seaisland cotton rootstock and grafting by making a triangular incision. All above approaches are key measures for improving the survival rate of transplanted cotton seedlings.