钾水平对人参根叶保护酶、活性氧代谢及膜脂过氧化作用的影响
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S567.5+1

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国家自然科学基金(3150111227);吉林省科技发展计划项目(20140204056YY);中央级公益性科研院所基本科研业务费专项(1610342016018、1610342016010)


Effects of Potassium Levels on the Protective Enzyme Activities,Active Oxygen Metabolism and Lipid Peroxidation in Roots and Leaves of Panax ginsneg C. A. Meyer
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    摘要:

    研究钾水平对人参根系、叶片保护酶活性及膜脂过氧化作用的影响。利用水培的方法,考察0.0 mmol/L,3.0mmol/L,6.0 mmol/L,12.0 mmol/L钾水平对人参根和叶保护酶、活性氧代谢及膜脂过氧化作用的影响。结果表明不同钾水平条件下,保护酶系统对钾水平响应的趋势不同。除叶片中SOD活性一直呈现显著上升趋势外,人参根系保护酶SOD、根叶保护酶POD活性均随着钾水平的升高呈现出先升高后下降的趋势,3.0 mmol/L的条件下活性最强,后期主要表现为被动忍耐。根系SOD、CAT和POD活性小于叶片。CAT活性与SOD和POD趋势不同,呈现出先降低后又升高的趋势,即主要在缺钾和高钾条件下发挥作用,6.0 mmol/L条件下,CAT活性最低。膜脂过氧化产物MDA含量随钾水平的升高先降低之后升高,6.0 mmol/L条件下最低。根系MDA含量小于叶片MDA含量。不同钾水平条件下,SOD、POD、CAT保护酶相互协调作用共同抵御活性氧损伤,促进人参健康生长。

    Abstract:

    Effects of potassium on protective enzyme activities, active oxygen metabolism and lipid peroxidation in roots and leaves of of Panax ginsneg C. A. Meyer were studied by hydroponics method. Four potassium levels of 0.0 mmol/L, 3.0 mmol/L, 6.0 mmol/L, 12.0 mmol/L were investigated. Results showed that the responses of protective enzyme system under the different potassium levels were different. The SOD activities of leaves in the P. ginsneg showed an increasing tendency. The activities of SOD(root), POD(roots and leaves) in the P. ginsneg increased at first, and then decreased with the increase of potassium levels, the highest activities reached at 3 mmol/L, and then presented as passive tolerance. The activities of SOD, CAT and POD in the leaves were significantly greater than those in the roots. The CAT activities were entirely different from SOD and POD, it decreased with the increase potassium levels until 6.0 mmol/L, and then increased. CAT played the mainly role under the deficient potassium levels and high potassium levels. MDA produced by membrane lipid peroxidation decreased with the increase of potassium concentration until 6.0 mmol/L, and then increased. Protective SOD, POD and CAT enzymes coordinated with each other to resist reactive oxygen species and promote healthy growth.

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刘政波,张春阁,刘宁,关一鸣,李乐,孙海,王秋霞,李美佳,张亚玉.钾水平对人参根叶保护酶、活性氧代谢及膜脂过氧化作用的影响[J].东北农业科学,2017,42(3):9-13.

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  • 收稿日期:2017-03-17
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  • 在线发布日期: 2024-12-06
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