By taking T. chilonis and T. dendrolimi propagated with Antheraea pernyi host eggs as the research object,the development duration, development threshold temperature and effective accumulated temperature of two Trichogramma Species were compared. The results showed that development duration of each life stage of two Trichogramma Species were significantly different at temperature 17-33℃, and theirs development duration of generation were different also. The rate of generation of T. chilonis y=0.2079-2.9390/x, the rate of generation of T. dendrolimi y=0.2183-3.1482/x. The results showed that developmental threshold temperature of each life stage of two Trichogramma Species were different. Development threshold temperature of eggs of T. chilonis was 7.85℃, which was higher than T. Dendrolimi, 5.10℃. Effective accumulated temperature of two Trichogramma Species was different. T. chilonis was 174.19 day-degree and T. dendrolimi was 183.33 day-degree. The development duration of each life stage of two Trichogramma Species had been calculated. This conclusion is of significance for better application of two Trichogramma Species in the future.