214 strains of Fusarium spp. were isolated in 13 cities and counties of the main producing areas of rice,wheat and maize in Heilongjiang, Jilin and Liaoning provinces. Of which, the highest isolation frequency was Fusarium graminearum（58.1%）. Fusarium spp. produces zearalenone（ZEN）, which has a strong biological toxicity and makes a strong poisoning effect to humans and animals. Therefore, the detection of zearalenone content in crops is very necessary. In this paper, ZEN-BSA conjugated to bovine serum albumin（ZEN-BSA） was used as antigen to immunize mice. The results of specific analysis for monoclonal antibodies showed that the binding rates of anti-ZEN monoclonal antibody to ZEN, α-zeranol, β-zeranol and β-zearalenol were 100%, 188.7%, 43.9% and 25.6%, respectively. And the binding rate to deoxynivalenol and aflatoxin was less than 1.0%, which proved that the monoclonal antibody prepared by the experiment had higher specificity. The monoclonal antibody was prepared for ELISA detection method based on the model, the detection limit is 0.4 ng/m L, the detection range of 0.4-25.00 ng/m L, the curve regression equation is y=-0.7673 x+3.1318, and the cross reaction of similar toxins were less than 1%, the recovery was 79%-112%. The range of toxin production with ELISA was 0.27-1.61μg/L. There were 6 strains of Fusarium graminearum toxin producing strains with ability. The results showed that the indirect competitive ELISA method can be used for the detection of zearalenone.