The objective of this study was to establish the conditions of ultrasonic assisted extraction of polysaccharides from Polygonatum odoratum. The optimum conditions were obtained by using single factor experiments and orthogonal experiment. The results showed that the optimum conditions of ultrasonic assisted extraction of polysaccharides from Polygonatum odoratum were as followings: liquid-solid ratio of 25∶1, ultrasonic time of 12 min, and ultrasonic temperature of 55°C, while extraction yield of polysaccharides from Polygonatum odoratum was 9.08%.