The difference in nutrient composition between transgenic foods and their parents is one of the important indicators for measuring the safety of genetically modified foods. In this study, based on the preliminary analysis of the transgenic corn HiII-NGc-a with compound trait of insect and herbicide resistance, the nutrient contents of the transgenic corn seeds containing the target genes cry1 Ab/Gc, bar and their parent non-transgenic corn were analyzed, including moisture, fat, ash, crude fiber, protein, amino acids, fatty acids, minerals, vitamins and anti-nutritional factors. The results showed that the nutrient contents of transgenic corn and non-transgenic corn were similar,and the results of most nutrients determination were within the normal reference range. The transgenic corn and non-transgenic corn had substantial equivalence.