Peanut（Arachis hypogaea L.） is the major economy crop and oil crop of Jilin Province, which located at the northeast high altitudes early-maturing area. There was over 267 thousand ha in Jilin Province, and low temperature affected growth and development of peanut. Chilling is the major abiotic stress for peanut in Jilin Province. This research was focus on identification of cold-resistance during the germination and the seedling periods of peanut using RGP and EA as index. Through the evaluation index, cold resistance during germination period was evaluated 1-3 grade and seedling period was evaluated 1-3 grade. That was foundation for selection of specific germplasm of cold resistance and breeding new cold resistant varieties.