In order to further analyze the salt-tolerant maize inbred lines, morphological observation and physiological detection were used in this study. The results showed that the height of 4 inbred lines decreased after 7 days of soda alkali stress, only ‘Zheng 58’expressed significantly. Moreover, the chlorophyll content of ‘Zheng 58’and ‘WD Zheng 58’increased significantly, the chlorophyll content of ‘Mei-2’increased but not significantly. In addition, the chlorophyll content of black maize decreased slightly but not significantly. Ultimately, the MDA content of the four inbred lines increased but not significantly; the content of proline and soluble sugar, the activity of POD did not increase significantly. The results implied that the stress intensity or stress time may be not enough to cause serious damage to the cell membrane structure, and the cell did not produce excessive osmotic regulators and protective enzymes to resist stress. The results can provide reference for identification maize inbred lines under soda alkali stress in the future.