In order to detect mosaic virus of soybean rapidly and effectively, we established indirect ELISA detection method by using prepared monoclonal antibody of soybean mosaic virus and optimizing the influence factors.The antigen was coated onto the microtiter plates at 37℃ for 1 h. The detection antibody at 125 ng/mL was incubated for 1 h. The enzyme labeled antibody at 100 ng/mL was incubated for 30 min. The detection sensitivity was 3.23 ng/mL for smv cp protein. The coefficient of variation of reproducibility was less than 3%. when the developed indirect ELSIA method was compared with RT-PCR, the concordance rate was 94%. Establishment of indirect ELISA for soybean mosaic virus coat protein laid the foundation for development of detection kit and test strip.