African swine fever outbroke in China in 2018. The p30 gene（GenBank No. MK482370） was cloned from an African Swine Fever Virus（ASFV） strain isolated in Shenyang. It has 100% nucleotide identity with Anhui isolated strain（GenBank No. MK128995）. However, there is some difference with reported SY-18（GenBank No.MH766894）. It has 585 base pairs（bp） coding 194 amino acids（aa）, while the p30 gene of SY-18 has 606 bp coding201 aa. This observation suggested that variation occurred in ASFV of China, whether the virulence varies remains to be further studied. Plasmid pGEX-6 p-1 were used in this study to express the hydrophilic area of p30 gene, and the recombinant proteins were purified by Ni-NTA-agarose affinity chromatography. This study provided antigen for ASFV serologic surveillance.