The detoxification seedlings of potato cultivars ‘Atlantic’, ‘Chunshu 3’, ‘Chunshu 5’and ‘Jishu 1’were used as experimental materials, and the conditions of their stem tissue regeneration were explored and optimized with an aim to establish an efficient regeneration protocol in potato. The results showed that ‘Atlantic’exhibited advantages over the 4 cultivars tested in terms of callus induction and differentiation. Under our experimental condition, Atlantic’s stem tissue sprout quickly and differentiated into callus of bright green with an induction rate and differentiation rate of 100% and 95%, respectively. The differentiation rates of other 3 cultivars were estimated to be 79.4% for ‘Chunshu 3’, 68.75% for ‘Chunshu 5’and 85.7% for ‘Jishu 1’. Using the MS medium supplemented with 2.0 mg/L 6-BA + 0.5 mg/L 2, 4-D + 0.4 mg/L KT, the stem tissue of the 4 potato cultivars may be differentiated completely into callus within 20 days.