In order to screen probiotics with assistant hypoglycemic function, a type 2 diabetes mouse model induced by streptozotocin combined with high-fat diet was built. The lactobacilli independently isolated and identified by us were given to type 2 diabetes mice by gavage for continuous 4 weeks. The body weight and fasting blood glucose levels were measured and the lipide, insulin, and inflammatory factors levels in serum were also measured so as to screen new strains with assistant hypoglycemic effects. The results showed that Lactobacillus plantarum S2（L.plantarum S2） could significantly reduce blood lipid-related indexes（including, fasting blood glucose, total serum cholesterol, triglycerides, free fatty acids, low-density lipoprotein cholesterol）, and inflammatory cytokines levels（including tumor necrosis factor-α, interleukin-6 C-reactive protein）, but significantly increased serum high-density lipoprotein cholesterol and insulin levels. Above results indicated that L. plantarum S2 plays an assistant hypoglycemic effect.