A total of 28 strains of four Trichogramma species were used to test the parasitic rate and eggs laying with the eggs of Chilo suppressalis in the laboratory. The results showed that the parasitic effect of S-AC strain of T. dendrolimi, M-TC strain of T. chilonis and D-JN strain of T. japonicum were better. The control efficacy were obtained through the release of single and mixed strains in the field. The effect of T. japonicum was better than that of T. chilonis, and T. chilonis was superior to T. dendrolimi. The effect of releasing mixed strains to control Chilo suppressalis was better than that of T. dendrolimi and T. chilonis alone. The average control effect of the release of the mixed strain was about 75%, which has a wide application prospect.