The effects of Gongzhulingmycin on the primary growth indexes of rice germination rate and seedling establishment were studied. The differences among the growth indexes in period of breeding rice under the different cultivation modes with Gongzhulingmycin in laboratory simulation conditions were also tested. The optimum dosages of different application methods were preliminarily determined. Its’ practicability during the period of rice sprout cultivation was assessed synthetically based on data of the seeding performance and the impact from diseases. The results showed that Gongzhulingmycin could increase germination rate and height of seeding when it was applied in the rice seedling stage. It could inhibite the root elongation slightly, but there was no significant effect on seeding fresh weight and root fresh weight. 2 000 times dilution treatment could promote plant height significantly, which was 26.09% higher than the control of water and 17.57% higher than the 1 000 times dilution treatment of brassatrol lactone. The optimum dosages were variety among the three different application methods of soaking, spraying and mixing soil. The optimum ratio of Gongzhulingmycin soil mixing was 1∶1 000-1∶1 500. The optimum concentration for soaking seeds was 500 times dilution. The optimum concentration of spraying after sowing was 2 000 times dilution. The optimum application concentration of Gongzhulingmycin in greenhouse was slightly higher than the laboratory simulation conditions. The application of Gongzhulingmycin in the rice seedling stage had a certain effect on controlling of bakanae disease, and the highest control effect against bakanae disease was 65.50%.