The purification process of Proanthocyanidins from hawthorn was studied in this paper. Static adsorption tests were carried out on six different types of resins to determine the best resin for purification. The effects of six factors on the adsorption rate of Hawthorn proanthocyanidins were investigated: concentration of sample solution, adsorption rate, concentration of desorption solution, desorption rate, desorption end point and pH value of column solution. The optimum conditions for purification of Hawthorn proanthocyanidins by macroporous resin were as follows: adsorption rate 3 BV/h; concentration of column solution 20.08μg/mL; pH=3; concentration of desorption solution 95%; desorption rate 1 BV/h; desorption amount 56 mL ethanol; The purity of Hawthorn proanthocyanidins increased from 7.42% to 34.39%.