The third generation of subculture seedlings of ‘Red Double Delight’ Chinese rose were used as experimental material. The orthogonal test was carried out to study effect of the refining time,the cutting matrix,the air humidity and different nutrient solution on the rooting of ‘Red Double Delight’ Chinese rose ex-vitro,in order to establish the rooting technology of ‘Red Double Delight’ Chinese rose ex-vitro. The results showed that the optimal culture conditions for the ex-vitro rooting were as follows: after the seedlings were planted indoors for 3 days,the cuttings were placed in a matrix（fine sand∶Vermiculite∶Perlite = 1∶1∶1） mixed with rooting nutrient solution（1/6 MS + 0.05 mg/L IBA + 0.10 mg/L NAA）. At the same time,the air humidity was maintained at 90% RH,and the rooting rate outside the bottle could reach 88.89%. The factors affecting the ex-vitro rooting rate were according to the order: the cutting matrix ＞ the time of refining ＞ the air humidity. In the rooting nutrient solution,the components affecting the ex-vitro rooting rate were in such a order: medium mother liquor ＞ IBA ＞ NAA.