This study compared The effect of 5 bio-organic fertilizers on the yield, agronomic traits and physiological characteristics of mung bean was compared in the study to select the best fertilizer in order to increase the yield,improve the efficiency of the mung bean production and promote the green production mode. The results showed that the effect of composite microbial agent was significant, plant fresh weight was 172.0 g, plant dry weight was 45.7 g,the fresh weight of plant above ground part was 164.0 g, the dry weight of above ground part reached 40.0 g, the number of nodules was 18.8, nodule fresh weight was 0.20 g and the dry weight of the nodules was 0.05 g. The highest yield was 2 077.3 kg/ha and 1 938.3 kg/ha,which was in TBK composite microbial original bacteria and composite microbial agent treatment. Therefore, TBK composite microbial original bacteria and composite microbial agent can effectively promote the growth and development of mung bean, stimulate the relevant physiological and biochemical reactions, significantly enhance the yield of mung bean.