A drought regulatory gene from arabidopsis thaliana, DREB2A, was transformed into the ‘Liao 5 buckwheat’using agrobacterium-mediated method to improve its drought tolerance. The factors influencing agrobacterium transformation were analyzed by the orthogonal test. The transformation system was established and optimized.The results showed that: 1. The cotyledons were selected as callus induction receptors during callus induction. The ratio of the hormones in the induced callus medium was that the concentration of 2,4-D was 2 mg/L, and the concentration of 6-BA was 1 mg/L. 2. During the transformation of agrobacterium, the optimum agrobacterium OD₆₀₀ was 0.5, the infection time was 3 min, the co-culture time was 3 days, the concentration of acetosyringone was 100 mg/L,the concentration of carbenicillin was 50 mg/L, the concentration of phosphinothricin was 100 mg/L.3. The PCR results showed that the gene DREB2A had been transformed into the ‘Liao 5. buckwheat’. 4. The physiological test of the transformed plants showed that the modified buckwheat had higher drought resistance than the non-transgenic.