Phytophthora root and stem rot caused by Phytophthora sojae is a destructive disease in soybean production regions in the world. To plant resistant variety is the most effective method for controlling the disease, and utilization of GMO play a major role in disease resistance breeding. Total 280 soybean GMO from Jilin Academy of Agriculture Sciences were evaluated by inoculated at hypocotyl using Physiologic Race 1 of Phytophthora sojae, which is the superiority race in Jilin Province. The results showed that the resistant germplasms were 27, which accounted for 9.6%; the susceptible germplasms were 188, which accounted for 67.1%; and the medium germplasms were 65,which accounted for 23.2%, respectively.