In order to screen out the suitable bacteria for fresh chicken manure compost fermentation, three bacteria and blank control were selected. A total of four groups were arranged and the water content, ammonia gas, hydrogen sulfide content and temperature changes in the pre-fermentation period detected. Research results showed that the temperature change in treatment adding fermentation agent C was significantly higher than that of the other three groups（P＜0.01）. The water content of the four groups changed the same way, i.e., rising, falling, rising and falling again. The water content in treatment 3 was significantly lower than control group（P＜0.05）. The content of ammonia and H₂ S in the four treatments showed a gradually decreasing trend, and they decreased in treatment with bacteria compared with the control, but the difference was not significant（P＞0.05）. The results showed that the Agent C is suitable for extension and application.