It is common for seed production by gynoecious inbred line in Cucurbitaceae melon crops, and how to keep female line is also an important research topic for breeders. It is an important method of keeping female line to induce male flower in a specific period of seedlings. In this paper, some factors influencing male flowers induction of the gynoecious plants were reviewed, including the age of seedling, types of plant growth regulator, concentrations of regulator, times of treatment and seasons of cultivation, etc. Some suggestions were put forward to provide references to the future staminate flower-inducing in gynoecious plants and Cucurbits gender experiments.