21份玉米单倍体诱导系遗传背景及诱导率分析
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S513

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吉林省农业科学院创新工程项目(CXGC82018ZY017、C92071513);吉林省农业科技创新工程项目-博士后基金(c92071510)


Analysis of Genetic Background and Inducing Rate of 21 Haploid Inducers
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    摘要:

    为明确改良和应用不同血缘背景诱导系,本研究选用21个外引及自选诱导系,采用基于Geno Plexs多重PCR的1K SNP标记进行基因分型并应用层次聚类法进行类群划分,同时以先玉335和3份糯玉米杂交种为母本进行杂交诱导,用以评判诱导系的诱导能力。结果表明,21份诱导系被划分为3类血缘,分别是Stock6为代表的类群Ⅰ、吉诱101代表的类群Ⅱ和农大高诱系列代表的类群Ⅲ;类群Ⅱ平均诱导率显著高于类群Ⅲ和类群Ⅰ,自选系WY7在选育过程中相比于背景诱导系产生了诱导率增益,WY7和W7-4可用于诱导糯玉米。

    Abstract:

    In order to improve and apply the haploid inducers of different blood backgrounds clearly, in this study,21 introduced and self-selective haploid inducers were selected, and Geno Plexs multiplex PCR-based 1 K SNPmarkers were used for genotyping and hierarchical clustering was used for group classification.’Xianyu 335’ and 3 waxy hybrids were used as a female parent to induce hybridization to judge the inducing ability of the haploid induc-ers. The results showed that 21 induction lines were classified into three types of blood, among which group Ⅰ repre-sented by Stock6, group Ⅱ represented by ‘Jiyou 101’, and group Ⅲ represented by the CAUHO. The average in-ducing rate of group Ⅱ was significantly higher than group Ⅲ and group Ⅰ. The self-selected lines,’Jiyou 101’ andWY7 produced an induction rate gain compared to the background haploid inducers during the selection process.WY7 and W7-4 can be used to induce waxy corn.

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王薪淇,岳尧海,董亚琳,孟令聪,路明,周德龙,孙传波.21份玉米单倍体诱导系遗传背景及诱导率分析[J].东北农业科学,2019,44(6):10-13,32.

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  • 收稿日期:2019-04-01
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  • 在线发布日期: 2024-12-04
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