The objective of this study was to compare the effects of corn stalk compressed matrixes on growth and nutrient elements accumulation of tomato and chili seedling. For determination of the composition of compressed matrix, to provide the basis for the utilization of corn stalk to seedling substrate. By measuring the fundamental properties of compressed matrix and observing the growth indexes and physiological indexes and nutrient elements accumulating of tomato and chili seedling grown on the matrix, the effect of matrix composition on seedling growth and nutrient elements accumulation were analyzed. Results showed that compressed matrix with fermented corn stalk were more suitable for seedling growth and nutrient elements accumulating than raw corn stalk. All indexes of two variety seedling grown on the prepared matrixes got the similar changing rule. Matrix T3（fermented corn stalk∶vermiculite = 2∶1） got the best germination percentage, seedling index, root vitality, chlorophyll content and nutrient elements accumulating, which were better than control matrix. In conclusion, the compressed corn stalk matrix could be utilized for seedling cultivation.