The technology of potato meristem detoxification is the most effective method to prevent the virus invasion and to solve the degradation of potato. To maintain the quality of the new potato variety ‘Jishu 1’, the meristem of the new potato variety ‘Jishu 1’ was were used as explants,the different disinfection methods and the tissue rapid propagation medium were optimized in this study. The potato mertem were first sterilized in 0.1% HgCl₂ for 8 min and 75% alcohol for 30 s, and then MS+6-BA 2 mg·L^-1+GA₃0.2 mg·L^-1+NAA 0.2 mg·L^-1 medium were used for meristem induction,and MS+PIX 1.0 mg·L^-1 medium were used for subculture,these can meet the factory demand of reproduction.