In this paper,"Wuyoudao 4", a high quality rice variety, was used as the experimental material to form a 2～4 row transplanting mode by changing the plant, row spacing and transplanting direction. From the perspective of introducing side row effect and improving the ventilation and light environment, the effects of transplanting mode and planting density on the cultivation yield and rice quality of organic rice were studied. The result showed that the treatment with the highest yield was transplanting rice seedlings with equal spacing（60.0 cm+30.0 cm+30.0 cm+30.0 cm）×13.3 cm in the 4-row group,which increased the yield by 12.32% compared with that of 30.0 cm×20.0 cm（16.67 holes/m²）with the control group. By increasing the number of transplanting holes per unit area, reducing the number of tillers per hole, and increasing the proportion of main panicle on the premise of ensuring the number of panicle per unit area, which was conducive to improving the full grain rate and rice quality. In the 2-row group（50.0 cm+30.0 cm）×20.0 cm and（50.0 cm+30.0 cm）×13.3 cm（12.50 holes/m²;18.80 holes/m²）, the grain plumpness increased because both rows were in the side row position, which was helpful to improve rice quality.