In order to establish a rapid, efficient, stable and high quality chicken blastoderm DNA extraction method, the X stage chicken blastoderm were collected and extracted by phenol chloroform extraction method,Kit Method of DNA extraction from micro tissue and optimized silicone membrane adsorption method respectively. The stability and quality of the extracted DNA were preliminary evaluated by agarose gel electrophoresis of 0.8% and further analyzed by PCR. The results showed that the genomic DNA extracted by Kit Method of DNA extraction from micro tissue was more stable and better quality than that extracted by phenol chloroform extraction method, while the stability and quality of genomic DNA extracted by Kit Method of DNA extraction from micro tissue was similar to that of the optimized silicone membrane adsorption method. However, the optimized silicone membrane adsorption method was established, which did not need protease K to digest the blastoderm tissue, so the steps were optimized and the cost was reduced. The results showed that a simple, efficient and stable DNA extraction method with high-quality and low-cost for X stage chicken blastoderm were established and it can be used in subsequent related studies.