The MYB family is the most important member of transcription factors that regulate anthocyanins synthesis. Through early transcriptome sequencing, it was found that MYB108 gene in the MYB transcription factors affecting anthocyanins had different expression in different periods, and its regulation effect on anthocyanin biosynthesis was not reported. The MYB gene was successfully cloned and identified as a MYB108 transcription factor family gene with an ORF of 891 bp, pI of 6.30, containing 296 amino acids, and it’s an unstable protein with a SANT/Myb domain. The color of red pear was affected by the anthocyanin content. The purpose of the study was to provide a prerequisite for further verification of the relationship between the gene and the anthocyanin.