酶解豆粕制备鲜味肽外切酶筛选与工艺优化
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

TS229

基金项目:

大豆产业技术体系(CARS-04-PS29);典型农产品品质指标体系构建与特征性指标筛选验证项目(GJFP2019043)


Screening and Process Optimization of Umami Peptides Exonuclease for Enzymatic Hydrolysis of Soybean Meal
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    本试验以高温豆粕为原料,以水解度和滋味稀释因子为筛选指标,首先比较了外切酶Flavourzyme和复合蛋白酶的酶解效果,筛选出最佳外切酶为Flavourzyme。再采用单因素试验考察该酶的最适添加量、酶解pH和酶解时间。最后,通过L9(34)正交试验确定了Flavourzyme酶解高温豆粕的最佳工艺组合,即加酶量22 U/g,pH 5.8,酶解时间2.5 h,在此条件下豆粕蛋白水解度高达27.80%。

    Abstract:

    In this experiment, high temperature soybean meal was used as raw material, hydrolysis degree and taste dilution factor were used as screening indexes. Firstly, the enzymatic hydrolysis effect of exonuclease Flavourzyme and complex protease was compared, and the best exonuclease was selected as Flavourzyme. Secondly, the single factor test was used to investigate the optimum enzyme concentration, pH and hydrolyzing time of hydrolysis. Finally, the best technology combination of Flavourzyme hydrolysis of high temperature soybean meal was determined by the L9(34) orthogonal test, that is, adding 22 U/g of enzyme, pH 5.8, enzymatic hydrolysis time 2.5 h, under this condition, the degree of protein hydrolysis of soybean meal reached 27.80%.

    参考文献
    相似文献
    引证文献
引用本文

范海茹,许斌,高雅鑫,张蒙冉,王凤忠,李淑英.酶解豆粕制备鲜味肽外切酶筛选与工艺优化[J].东北农业科学,2020,45(2):111-115.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-09-09
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2024-11-26
  • 出版日期:
文章二维码