Soybean root rot, Phytophthora and cyst nematode are the major soil-borne diseases that are extremely difficult to control. Planting resistant varieties are effective measures, and screening of resistant germplasm is the basis of breeding resistant varieties. 76 soybean materials were inoculated with Fusarium oxysporum, race 1 of Phytophthora sojae and race 3 of cyst nematode respectively. The resistance of each soybean germplasm was evaluated according to the resistance evaluation standard of each disease. A total of 11 anti-root rot materials, 13 anti-Phytophthora materials, and 9 materials with moderately resistant soybean cyst nematode were screened out. 6 varieties（lines） were resistant to two diseases, accounting for 7.9%. Only 1 material was resistant to the above three diseases,accounting for 1.3%. The results will provide a theoretical basis for the cultivation of new varieties and Identification of disease resistance genes.