In order to clone the Keratinocyte-associated protein 2（KRTCAP2） gene and identify the expression pat-tern in different organs of sheep, in this study, the total RNA was extracted from different organs coming from Smalltail Han sheep（STH sheep） and Xinji Fine wool sheep（XJ sheep）, and KRTCAP2 was cloned by RT-PCR, the polymorphisms in different sheep populations were tested by HRM. In the end, the tissue expression pattern was measured by q RT-PCR. The RT-PCR amplification showed there was a specific band which was slightly smaller than 500 bp, and the sequencing data approved the amplicons were KRTCAP2 gene with 466 bp in length encoding 149 amino acids peptides. In the same time, four SNP mutants were identified, and two of them caused mutation in protein. The polymorphism of A＞G mutant in c.194 by HRM analysis showed there were no polymorphism in all populations including STH sheep and XJ sheep. The tissues expression distribution analysis by q RT-PCR approved that KRTCAP2 gene was constructive expressed in all organs in sheep. However, the expression pattern has both similarand distinct point in organs from STH sheep and XJ sheep. The similar point is KRTCAP2 has higher expression inliver, spleen, lung, kidney, gut and skin than heart in both STH sheep and XJ sheep, The expression levels in liver,spleen, lung, intestine, brain and skin were higher in XJ sheep than in STH sheep. The expression in muscle and ovary tissue of XJ sheep was lower than that of STH sheep, and the expression in kidney tissue was lower. In conclusion, the sheep KRTCAP2 gene was cloned in this study which provides an opportunity to explore the gene functionin further.