Soaking seeds plays an important role in rice production in cold regions. Providing suitable water, temperature and oxygen environment for rice seeds through artificial measures can promote seed germination. Its effect is related to seedling quality and rice yield. Microporous aeration is an effective method to realize gas-liquid two-phase oxygen mass transfer. In order to obtain the effect of oxygen environment on seed germination and the law of seed oxygen demand during rice seed soaking in cold region, the experiment of microbubble aeration with different frequencies was carried out on rice variety Dongnong 426 in cold region during seed soaking, and the law of oxygen consumption in seed soaking water was measured and the seed germination rate was compared. The results showed that seed germination rate was significantly increased by microbubble aeration soaking, and the germination rate in-creased with the increase of aeration frequency. According to the general law of oxygen consumption, seeds do not consume oxygen in 0～12 h of seed soaking, but slow in 12～25 h, dissolved oxygen consumption begins to accelerate at 25 h. After that, the oxygen consumption rate accelerated with the soaking time, and reaches the highest at the completion of seed soaking. Dissolved oxygen consumption rate was the same in 1～6 days with different increasing frequency, and increased with frequency in 7～9 days. In addition, the law of oxygen consumption in soaking water and the law of dissolved oxygen variation in clear water were compared and analyzed. The supersaturated dissolved oxygen could be produced by aeration, but it could not be considered in the process of seed soaking.