Wild emmer in Israel has many advantages, such as resistance to powdery mildew, stripe rust and leaf rust, rich in protein and selenium, drought tolerance, salt tolerance and high utilization rate of mineral nutrients.In this study FISH technique was applied to analyze karyotype characteristics of wild emmer from Israel, so as to provide reference for its application of this germplasm in wheat genetics and new variety cultivation. The results are as follow: using the Oligo-pAs1-1（red） and Oligo-pSc119.2-1（green）repeats as probes, the FISH analysis showed that here were 14 pairs of chromosomes in Israel wild emmer wheat inIsraelwild emmer wheat, and the oligo-pas1-1 red signal was mainly distributed on the a chromosome, while the bright and rich oligo-psc119.2-1 green signal was mainly distributed on the B chromosome. Through the distribution characteristics of these signals on chromosomes,each pair of chromosome can be accurately identified. The FISH karyotype of wild emmer wheat in Israel is different from that of common wheat such as Chinese Spring, which can be used to create new wheat germplasm.